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Output format

ApoplastP will return the output as shown in the example below. First, it will return the predicted apoplastic proteins in your set as FASTA sequences, if there are any. Second, a summary table will be shown which shows the predictions (apoplastic or non-apoplastic) for each submitted protein.

ApoplastP returns a probability that a tested instance will belong to either the apoplastic or non-apoplastic class and these probabilities are included in the web server output as additional information to researchers.

We deliberately did not recommend a probability threshold over which a protein would be classified as an apoplastic protein candidate, as we believe it should remain up to the individual user to interpret their results in the context of additional resources available. For example, a researcher might like to predict the full apoplastic candidate list and overlay this with in planta expression data to prioritize candidates, whereas in other situations without additional information a list of high-priority candidates as determined by the ApoplastP probabilities might be more appropriate.


Example output

2 of your 3 submitted proteins are predicted to be apoplastic (66.7%).


Predicted apoplastic proteins (download as FASTA file here):


>Ecp6| Apoplastic probability:0.96
MQSMILFAAALMGAAVNGFVLPRTDDPDCETKATDCGSTSNIKYTVVKGDTLTSIAKKFKSGICNIVSVNKLANPNLIELGATLIIPENCSNPDNKSCVSTPAEPTETCVPGLPGSYTIVSGDTLTNISQDFNITLDSLIAANTQIENPDAIDVGQIITVPVCPSSQCEAVGTYNIVAGDLFVDLAATYHTTIGQIKALNNNVNPSKLKVGQQIILPQDCKNVTTAVA
>AvrLm6| Apoplastic probability:0.78
MVIYLPLYLLVLGIATTTISQPHLLCACESGRRDGVDDTRTLKVVKGTGGRFVFSSRYWTKAEGAPHEGNYAHAINGTITKKGTNIQAHDDGLIGGEEMNSLCPEHSTCFSPNLKAKSTHSCGPDGKYGCVSAWLSVNWEGQIQ


Summary table


#IdentifierPrediction Probability
Ecp6Apoplastic0.96
AvrLm6Apoplastic0.78
AvrLm1Non-apoplastic0.51